Abstract
A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. The monoclinic crystals belonged to space group P2(1), with unit-cell parameters a = 46.8, b = 88.7, c = 49.9 A, beta = 111.9 degrees, and diffracted to 2.16 A resolution.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Crystallization
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Homeodomain Proteins / chemistry*
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Homeodomain Proteins / metabolism*
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LIM-Homeodomain Proteins
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Mice
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Motor Neurons / chemistry
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Motor Neurons / metabolism
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / metabolism
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Protein Binding / physiology
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Protein Engineering / methods
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Transcription Factors / chemistry*
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Transcription Factors / metabolism*
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X-Ray Diffraction*
Substances
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Homeodomain Proteins
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Isl2 protein, mouse
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LIM-Homeodomain Proteins
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Lhx4 protein, mouse
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Nerve Tissue Proteins
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Transcription Factors
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insulin gene enhancer binding protein Isl-1