Expression of cell cycle biomarkers and telomere length in papillary thyroid carcinoma: a comparative study between radiation-associated and spontaneous cancers

Am J Clin Oncol. 2009 Feb;32(1):1-8. doi: 10.1097/COC.0b013e3181783336.

Abstract

Objective: Radiation exposure during childhood is the only well-established risk factor for papillary thyroid carcinoma (PTC). To better define the biologic profile of radiation-induced and sporadic PTC, we compared in these two groups of PTC the expression of cell cycle regulatory proteins and telomere length.

Methods: Cell cycle markers (cyclin A, B1, D1, E, and Ki67) were evaluated on 100 PTC specimens (26 radiation-induced and 74 sporadic PTCs). The expression of cell cycle regulators was studied using immunohistochemistry; telomere length heterogeneity was studied using in situ hybridization in a subset of 16 formalin-fixed samples (8 radiation-induced and 8 sporadic PTCs).

Results: At multivariate analysis, only cytoplasmic cyclin E staining was overexpressed in sporadic cases (P = 0.006). The other cell cycle markers and telomere length did not differ significantly between sporadic PTC and radiation-induced PTC.

Conclusions: These markers cannot be used to differentiate radiation-induced from sporadic PTCs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma, Follicular / metabolism
  • Adenocarcinoma, Follicular / pathology
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Papillary / metabolism*
  • Carcinoma, Papillary / pathology
  • Case-Control Studies
  • Cell Cycle Proteins / metabolism*
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization, Fluorescence
  • Infant
  • Male
  • Middle Aged
  • Neoplasms, Radiation-Induced / metabolism*
  • Neoplasms, Radiation-Induced / pathology
  • Prognosis
  • Telomere / metabolism*
  • Thyroid Neoplasms / metabolism*
  • Thyroid Neoplasms / pathology
  • Tissue Array Analysis
  • Young Adult

Substances

  • Cell Cycle Proteins