Cytokine-activated human mesangial cells generate the neutrophil chemoattractant, interleukin 8

Kidney Int. 1991 Jul;40(1):86-90. doi: 10.1038/ki.1991.184.

Abstract

Human mesangial cells (MC) in culture, when stimulated by interleukin 1 alpha(IL-1 alpha) or tumour necrosis factor (TNF alpha), but not with lipopolysaccharide (LPS), express interleukin 8 (IL-8) mRNA, and both cell associated and extracellular IL-8. Dexamethasone treatment of mesangial cells induced partial inhibition of the release of extracellular IL-8, while cell-associated IL-8 and IL-8 mRNA were not significantly altered. We propose that the mesangial cell has a direct role in the initiation and propagation of inflammatory events within the glomerulus via the generation of the chemotactic cytokine IL-8.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Cytokines / pharmacology*
  • Dexamethasone / pharmacology
  • Extracellular Space / metabolism
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / metabolism*
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-8 / biosynthesis*
  • Interleukin-8 / genetics
  • RNA, Messenger / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Cytokines
  • Interleukin-1
  • Interleukin-8
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Dexamethasone