Genome-based expression profiles as a single standardized microarray platform for the diagnosis of bladder pain syndrome/interstitial cystitis: an array of 139 genes model

Int Urogynecol J Pelvic Floor Dysfunct. 2009 May;20(5):515-22. doi: 10.1007/s00192-009-0822-0. Epub 2009 Feb 13.

Abstract

Introduction and hypothesis: The aim of the study was to investigate the molecular signatures underlying bladder pain syndrome/interstitial cystitis (BPS/IC) using cDNA microarray.

Methods: Microarray gene expression profiles were [corrected] studied in a matched case-control study [corrected] by using a system of conditional regression modeling.

Results: The main [corrected] findings are summarized as follows: Firstly, a "139-gene" model was discovered to contain high expressions of bladder epithelium, which feature in BPS/IC. Secondly, complex metabolic reactions, including carbohydrate, lipid, cofactors, vitamins, xenobiotics, nucleotide, and amino acid metabolisms, were [corrected] found to have a strong relationship with bladder smooth muscle contraction through IC status. Thirdly, we [corrected] found the transcriptional regulations of IC-induced bladder smooth muscle contraction status, including the level of contractile force, tissue homeostasis, energy homeostasis, and the development of the [corrected] nervous system. In addition, our study suggested the mast-cell activation mediated by the high-affinity receptor of Fc epsilon [corrected] RI triggering allergic inflammation through IC status. Such genetic changes, jointly termed "bladder remodeling," [corrected] can constitute an important long-term consequence of BPS/IC. [corrected].

Conclusions: The success of this innovation has supported the use of microarray-based expression profiling as a single standardized platform for diagnosis of PBS/IC and offers [corrected] drug discovery.

MeSH terms

  • Actins / genetics
  • Actins / metabolism
  • Adipokines / genetics
  • Adipokines / metabolism
  • Amino Acids / metabolism
  • Apoptosis / genetics
  • Carbohydrate Metabolism / genetics
  • Case-Control Studies
  • Cells, Cultured
  • Cystitis, Interstitial / diagnosis*
  • Cystitis, Interstitial / genetics*
  • Cytokines / genetics
  • DNA, Complementary
  • Epithelial Cells
  • Female
  • Gene Expression Profiling*
  • Homeostasis / genetics
  • Humans
  • Lipid Metabolism / genetics
  • Logistic Models
  • MAP Kinase Signaling System / genetics
  • Muscle Contraction / genetics*
  • Muscle, Smooth / innervation
  • Muscle, Smooth / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Peroxisome Proliferator-Activated Receptors / genetics
  • Peroxisome Proliferator-Activated Receptors / metabolism
  • Transcriptome*
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism
  • Urothelium

Substances

  • Actins
  • Adipokines
  • Amino Acids
  • Cytokines
  • DNA, Complementary
  • Peroxisome Proliferator-Activated Receptors
  • Transforming Growth Factor beta