Treatment of experimental periodontal disease by photodynamic therapy in immunosuppressed rats

J Clin Periodontol. 2009 Mar;36(3):219-28. doi: 10.1111/j.1600-051X.2008.01355.x.

Abstract

Background and objective: The aim of this study was to compare photodynamic therapy (PDT) as an adjunctive treatment of induced periodontitis with scaling and root planing (SRP) in dexamethasone-inhibited rats.

Material and methods: The animals were divided into two groups: ND (n=90), saline solution treatment; D (n=90), dexamethasone treatment. In the ND and D Groups, periodontal disease was ligature-induced at the first mandibular molar. After 7 days, the ligature was removed and all animals received SRP and were divided according to the following treatments: SRP, saline solution; Toluidine Blue-O (TBO), phenothiazinium dye; and PDT, TBO and laser irradiation. Ten animals in each treatment were killed at 7, 15 and 30 days. The radiographic and histometric values were statistically analysed.

Results: In the ND and D Groups, radiographic analysis showed less bone loss in animals treated by PDT in all the experimental periods than SRP and TBO at 15 days (p<0.05). After a histometric analysis was carried out in the ND and D groups, the animals treated by PDT showed less bone loss in all periods than SRP and TBO after 15 days (p<0.05).

Conclusions: The PDT was an effective adjunctive treatment of induced periodontitis compared with SRP in dexamethasone-inhibited rats.

Publication types

  • Comparative Study

MeSH terms

  • Alveolar Bone Loss / etiology
  • Alveolar Bone Loss / pathology
  • Alveolar Bone Loss / prevention & control
  • Analysis of Variance
  • Animals
  • Dental Scaling*
  • Dexamethasone / immunology
  • Disease Models, Animal
  • Immunosuppressive Agents / immunology
  • Low-Level Light Therapy / methods
  • Male
  • Mandible / pathology
  • Periodontitis / complications
  • Periodontitis / immunology
  • Periodontitis / therapy*
  • Photochemotherapy / methods*
  • Photosensitizing Agents / therapeutic use*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Tolonium Chloride / therapeutic use*
  • Treatment Outcome

Substances

  • Immunosuppressive Agents
  • Photosensitizing Agents
  • Tolonium Chloride
  • Dexamethasone