A nutrigenomic inflammation-related PBMC-based approach to predict the weight-loss regain in obese subjects

Ann Nutr Metab. 2009;54(1):43-51. doi: 10.1159/000205319. Epub 2009 Feb 27.

Abstract

Background: Long-term maintenance of a dietary-induced weight loss continues to be a major health problem and warrants research on innovative approaches to understand weight stability. We investigated the role of the proinflammatory status on weight changes in obese subjects receiving a low-calorie diet (LCD) and during the subsequent 6-month weight maintenance period.

Methods: Eighty-four subjects (age: 34.2 +/- 0.53 years; body mass index, BMI: 30.4 +/- 1.8 kg/m(2)) followed an 8-week LCD intervention and were contacted again 6 months later. Body composition, circulating proinflammatory markers [tumor necrosis factor (TNF)alpha, interleukin-6, C-reactive protein and leptin] and mRNA levels of inflammation-related genes [TNFalpha and nuclear factor (NF) kappaB transcription subunits] in peripheral blood mononuclear cells (PBMC) were evaluated.

Results: The 6-month weight regain was predicted by high concentrations of TNFalpha at LCD completion (OR = 4.21, p = 0.036) along with the baseline amount of fat mass (OR = 7.23, p = 0.029). In addition, baseline leptin concentrations (p = 0.028) as well as mRNA levels of TNFalpha and NFkappaB subunits were higher at the end of the dietary intervention (p < 0.05) in PBMC of subjects who regained >or=10% of the dietary-induced weight loss.

Conclusions: These findings demonstrate a role for the proinflammatory state and body adiposity in the prediction of weight-loss regain. This relationship could contribute to the design of more personalized nutritional treatments in obese patients and favor the weight maintenance process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • C-Reactive Protein / metabolism
  • Female
  • Gene Expression Profiling
  • Humans
  • Inflammation / blood
  • Inflammation / physiopathology*
  • Interleukin-6 / blood
  • Leptin / blood
  • Leukocytes, Mononuclear / pathology
  • Leukocytes, Mononuclear / physiology*
  • Male
  • NF-kappa B / blood
  • Nutrigenomics
  • Obesity / blood*
  • Obesity / physiopathology
  • Predictive Value of Tests
  • RNA, Messenger / metabolism
  • Tumor Necrosis Factor-alpha / blood
  • Weight Gain / physiology*
  • Weight Loss / physiology
  • Young Adult

Substances

  • Interleukin-6
  • Leptin
  • NF-kappa B
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • C-Reactive Protein