HPV genotyping from invasive cervical cancer in Chile

Int J Gynaecol Obstet. 2009 May;105(2):150-3. doi: 10.1016/j.ijgo.2008.12.017. Epub 2009 Feb 26.

Abstract

Objective: To determine the prevalence rates of the different HPV types in cervical cancer lesions in Chile to facilitate the development of prophylactic human papillomavirus (HPV) vaccines effective for that country.

Method: Biopsy samples of 312 cervical cancer lesions were assessed for HPV type by reverse-line blotting assay.

Results: HPV DNA was found in 94.2% of the lesions, 67.2% harboring 1 viral type and the remainder harboring more than 1 type. HPV-16 was the most frequent type in single infections (50.5%), followed by HPV-18 (7.8%), HPV-31 (2.4%), and HPV-45 (2.0%). HPV-16 was also present in 98.7% of dual and multiple infections, its most frequent association being with HPV-18.

Conclusions: HPV types 16, 18, 31, and 45, alone or combined with other types, were observed in the biopsy samples of up to 80.5% of cervical cancer lesions.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / epidemiology
  • Adenocarcinoma / virology*
  • Adult
  • Carcinoma, Squamous Cell / epidemiology
  • Carcinoma, Squamous Cell / virology*
  • Chile / epidemiology
  • DNA, Viral / analysis
  • Female
  • Genotype
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 16 / isolation & purification*
  • Human papillomavirus 18 / genetics
  • Human papillomavirus 18 / isolation & purification*
  • Humans
  • Middle Aged
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / virology*
  • Prevalence
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / virology*
  • Young Adult

Substances

  • DNA, Viral