Abstract
In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Actins / metabolism
-
Animals
-
Antibodies, Monoclonal / pharmacology
-
Cell Proliferation / drug effects
-
Cell Surface Extensions / drug effects
-
Cell Surface Extensions / enzymology
-
Cell Survival / drug effects
-
Cells, Cultured
-
Chemotaxis / drug effects
-
Cross-Linking Reagents / pharmacology
-
Cytoskeleton / drug effects
-
Cytoskeleton / enzymology*
-
Endocytosis / drug effects
-
Humans
-
Lipopolysaccharides / pharmacology
-
Macrophage Colony-Stimulating Factor / pharmacology
-
Macrophages / cytology*
-
Macrophages / drug effects
-
Macrophages / enzymology*
-
Mice
-
Mice, Inbred C57BL
-
Phosphorylation / drug effects
-
Protein Binding / drug effects
-
Protein Transport / drug effects
-
Receptor, Macrophage Colony-Stimulating Factor / metabolism
-
Receptor-Like Protein Tyrosine Phosphatases, Class 3 / metabolism
Substances
-
Actins
-
Antibodies, Monoclonal
-
Cross-Linking Reagents
-
Lipopolysaccharides
-
Macrophage Colony-Stimulating Factor
-
Receptor, Macrophage Colony-Stimulating Factor
-
Ptprj protein, mouse
-
Receptor-Like Protein Tyrosine Phosphatases, Class 3