Abstract
The histone variant H2A.Z (Htz1p) has been implicated in transcriptional regulation in numerous organisms, including Saccharomyces cerevisiae. Genome-wide transcriptome profiling and chromatin immunoprecipitation studies identified a role for Htz1p in the rapid and robust activation of many oleate-responsive genes encoding peroxisomal proteins, in particular POT1, POX1, FOX2, and CTA1. The Swr1p-, Gcn5p-, and Chz1p-dependent association of Htz1p with these promoters in their repressed states appears to establish an epigenetic marker for the rapid and strong expression of these highly inducible promoters. Isw2p also plays a role in establishing the nucleosome state of these promoters and associates stably in the absence of Htz1p. An analysis of the nucleosome dynamics and Htz1p association with these promoters suggests a complex mechanism in which Htz1p-containing nucleosomes at fatty acid-responsive promoters are disassembled upon initial exposure to oleic acid leading to the loss of Htz1p from the promoter. These nucleosomes reassemble at later stages of gene expression. While these new nucleosomes do not incorporate Htz1p, the initial presence of Htz1p appears to mark the promoter for sustained gene expression and the recruitment of TATA-binding protein.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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3-Hydroxyacyl CoA Dehydrogenases
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Acyl-CoA Oxidase / genetics
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Acyl-CoA Oxidase / metabolism
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Chromatin / metabolism*
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Enoyl-CoA Hydratase
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Gene Expression Regulation, Fungal*
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Histone Acetyltransferases / genetics
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Histone Acetyltransferases / metabolism
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Histones / genetics
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Histones / metabolism*
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Nucleosomes / metabolism
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Oleic Acid / metabolism*
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Promoter Regions, Genetic
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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TATA-Box Binding Protein / genetics
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TATA-Box Binding Protein / metabolism*
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Telomere-Binding Proteins / genetics
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Telomere-Binding Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Chromatin
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Histones
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Htz1 protein, S cerevisiae
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ISWI protein
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Nucleosomes
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Saccharomyces cerevisiae Proteins
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TATA-Box Binding Protein
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Telomere-Binding Proteins
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Transcription Factors
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Oleic Acid
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3-Hydroxyacyl CoA Dehydrogenases
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Acyl-CoA Oxidase
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POX1 protein, S cerevisiae
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GCN5 protein, S cerevisiae
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Histone Acetyltransferases
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Adenosine Triphosphatases
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Enoyl-CoA Hydratase
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FOX2 protein, S cerevisiae