Peroxisome proliferators-activated receptors (PPARs) plays important role in regulate a variety of target genes which involves in lipid metabolism. In the present study, 1,430 bp of PPARalpha and 1,650 bp of PPARgamma in Peking duck (Anas platyrhynchos) were cloned by reverse transcription (RT)-PCR. The cloned sequences of PPARalpha and PPARgamma were highly conserved, although relevant changes with respect to human, mouse, chicken, and goose were identified. Real-time quantitative RT-PCR was used to determine if the PPARalpha and PPARgamma were expressed in different tissues of male and female Peking ducks. The expression of PPARalpha and PPARgamma were widely distributed in all examined tissues. The expression level of PPARalpha was highest in liver and lowest in kidney in male and female ducks. And it was highest in abdominal fat and lowest in small intestine of PPARgamma in male and female ducks.