Abstract
Mice deficient in B cells (micromT mice) were used to evaluate the role of antibody in enhanced chlamydial clearance and reduction of pathology afforded by vaccination with recombinant chlamydial protease-like activity factor (rCPAF). Enhanced, but comparable, chlamydial clearance was observed in micromT and wild-type (WT) mice after rCPAF+CpG vaccination. Chlamydia-induced pathology was present in mock-immunized animals, but at significantly greater levels in micromT than WT mice, whereas vaccinated micromT and WT mice exhibited similar reductions in pathology. Thus, antibodies may play a role in protection against chlamydial pathology after primary infection, but were largely dispensable in rCPAF+CpG-induced chlamydial clearance and reduction in pathology.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / pharmacology
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Animals
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Antibodies, Bacterial / immunology*
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Antigens, Bacterial / immunology*
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Bacterial Vaccines / immunology*
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Chlamydia Infections / immunology
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Chlamydia Infections / microbiology
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Chlamydia Infections / pathology
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Chlamydia Infections / prevention & control*
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Chlamydia muridarum / immunology*
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Colony Count, Microbial
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Female
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Genital Diseases, Female / immunology
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Genital Diseases, Female / microbiology
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Genital Diseases, Female / pathology
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Genital Diseases, Female / prevention & control*
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Mice
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Oligodeoxyribonucleotides / administration & dosage
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Oligodeoxyribonucleotides / pharmacology
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Vaccines, Synthetic / immunology
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Vaccines
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CPG-oligonucleotide
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Oligodeoxyribonucleotides
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Vaccines, Synthetic