Interleukin-6 induces proinflammatory signaling in Schwann cells: a high-throughput analysis

Biochem Biophys Res Commun. 2009 May 1;382(2):410-4. doi: 10.1016/j.bbrc.2009.03.034. Epub 2009 Mar 11.

Abstract

Interleukin-6 plays an important role in peripheral nerve regeneration. We recently reported that IL-6 targets Schwann cells in the peripheral nerve for its function. In this study, we analyzed genes whose expression is regulated by IL-6 in a cell line derived from Schwann cells, the peripheral glia, using the Illumina gene microarray. At measurements 3 and 12h after IL-6 treatment, 35 genes were found to be upregulated by IL-6. Most upregulated genes were proinflammatory genes that are known to be induced in inflammatory conditions. Interestingly, the expression of immunoproteasome subunits was upregulated by IL-6 in Schwann cells. Treatment with forskolin, an agent that mimics axonal signaling, suppressed the expression of IL-6-inducible genes. Finally, we found for the first time that sciatic nerve injury induced immunoproteasome expression in vivo. These findings indicate that IL-6 is involved in peripheral nerve regeneration by regulating proinflammatory signaling in Schwann cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Colforsin / metabolism
  • Gene Expression Profiling*
  • Inflammation / genetics
  • Inflammation / immunology*
  • Interleukin-6 / pharmacology
  • Interleukin-6 / physiology*
  • Mice
  • Oligonucleotide Array Sequence Analysis
  • Rats
  • Schwann Cells / drug effects
  • Schwann Cells / immunology*
  • Sciatic Nerve / immunology
  • Sciatic Nerve / injuries
  • Signal Transduction / genetics
  • Signal Transduction / immunology
  • Tumor Necrosis Factor-alpha / metabolism
  • Up-Regulation

Substances

  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Colforsin