Abstract
In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / classification
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Aldehyde Oxidoreductases / genetics
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Aldehyde Oxidoreductases / metabolism*
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Mutation
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Nitrofurans / pharmacology
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Nitroreductases / classification
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Nitroreductases / genetics
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Nitroreductases / metabolism*
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Oligonucleotide Array Sequence Analysis
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Phylogeny
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / enzymology*
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Staphylococcus aureus / genetics
Substances
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Nitrofurans
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Aldehyde Oxidoreductases
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formaldehyde dehydrogenase, glutathione-independent
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Nitroreductases