Abstract
We used a well-characterized isogenic set of clinical bloodstream Staphylococcus aureus strains to study (i) regulation of mprF-mediated phosphatidylglycerol lysinylation in the contexts of in vitro daptomycin (DAP) nonsuceptibility and (ii) the role of mprF mutation in endovascular virulence. We observed a correlation between increased expression of a mutant mprF gene and reduced in vitro DAP susceptibility. There were no detectable fitness differences between strains in experimental infective endocarditis.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoacyltransferases / genetics*
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Aminoacyltransferases / physiology
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology
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Daptomycin / pharmacology*
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Drug Resistance, Bacterial
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Humans
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Microbial Sensitivity Tests
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Mutation
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Aminoacyltransferases
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mprF protein, Staphylococcus aureus
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Daptomycin