Abstract
The principal GTP-binding protein (G-protein) from squid (Loligo forbesi) photoreceptor membranes has been identified by amino acid sequencing. The heterotrimeric protein was purified by detergent solubilization and ion-exchange chromatography. The amino acid sequence of the G-protein alpha-subunit (G-alpha) indicates that this subunit is closely related to the recently characterized Gq subgroup, whereas the G-gamma subunit varies widely in sequence from other homologues.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Chromatography, Ion Exchange
-
Cyanogen Bromide
-
Decapodiformes
-
Detergents
-
Electrophoresis, Polyacrylamide Gel
-
GTP-Binding Proteins / chemistry
-
GTP-Binding Proteins / isolation & purification*
-
Iodobenzoates / chemistry
-
Molecular Sequence Data
-
Photoreceptor Cells / chemistry*
-
Sequence Homology, Nucleic Acid
-
Urea / chemistry
Substances
-
Detergents
-
Iodobenzoates
-
Urea
-
GTP-Binding Proteins
-
Cyanogen Bromide