Abstract
We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Exons*
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Genes
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Heart / physiology*
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Humans
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Molecular Sequence Data
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Myosins / genetics*
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Rats
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Sequence Homology, Nucleic Acid
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Transcription, Genetic
Substances
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Oligodeoxyribonucleotides
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DNA
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Myosins
Associated data
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GENBANK/M74785
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GENBANK/M74786
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GENBANK/M74787
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GENBANK/M74788
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GENBANK/M74790
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GENBANK/M81108
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GENBANK/M81109
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GENBANK/S57525
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GENBANK/S57535
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GENBANK/X56181