Barley variety identification using SSRs

Methods Mol Biol. 2009:508:309-17. doi: 10.1007/978-1-59745-062-1_24.

Abstract

There is a current and developing need for rapid and accurate methods of barley varietal identification which go beyond traditional morphological analysis. Methods using DNA analysis have the capacity to fulfil this role with microsatellites being the current marker of choice. The majority of barley cultivars on the National List can be differentiated using 6 SSRs and bulk samples, using the methods described here.

MeSH terms

  • Crops, Agricultural / genetics
  • DNA, Plant / analysis
  • Genetic Variation
  • Genome, Plant
  • Genotype*
  • Hordeum / classification
  • Hordeum / genetics*
  • Microsatellite Repeats / genetics*
  • Polymorphism, Single Nucleotide

Substances

  • DNA, Plant