Inhibitory effect of cadmium and mercury ions on transcription of the ada gene

M Suzuki; K Takahashi; Y Kawazoe; K Sakumi; M Sekiguchi

doi:10.1016/0006-291x(91)91744-w

Inhibitory effect of cadmium and mercury ions on transcription of the ada gene

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1517-21. doi: 10.1016/0006-291x(91)91744-w.

Authors

M Suzuki¹, K Takahashi, Y Kawazoe, K Sakumi, M Sekiguchi

Affiliation

¹ Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.

PMID: 1930191
DOI: 10.1016/0006-291x(91)91744-w

Abstract

The effect of Cd2+ and Hg2+ on transcription of the ada gene was examined using an in vitro system reconstituted with Ada protein and methylated DNA, together with their effect on methyltransferase activity of the Ada protein. Both Cd2+ and Hg2+ inhibited transcription of the ada gene over the doses which did not inhibit methyltransferase activity.

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Cadmium / pharmacology*
Escherichia coli / drug effects
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli Proteins*
Genes, Bacterial / drug effects*
Kinetics
Mercury / pharmacology*
Methyltransferases / genetics*
Methyltransferases / metabolism
O(6)-Methylguanine-DNA Methyltransferase
Transcription Factors
Transcription, Genetic / drug effects*

Substances

Bacterial Proteins
Escherichia coli Proteins
Transcription Factors
Cadmium
Methyltransferases
Ada protein, E coli
O(6)-Methylguanine-DNA Methyltransferase
Mercury