High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

Hongyan Sun; Lay Pheng Tan; Liqian Gao; Shao Q Yao

doi:10.1039/b817853d

High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

Chem Commun (Camb). 2009 Feb 14:(6):677-9. doi: 10.1039/b817853d. Epub 2008 Dec 12.

Authors

Hongyan Sun¹, Lay Pheng Tan, Liqian Gao, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, 3 Science Drive, Singapore 11754.

PMID: 19322419
DOI: 10.1039/b817853d

Abstract

We report herein a novel phosphopeptide microarray capable of noncovalently "trapping" catalytically inactive mutants of protein tyrosine phosphatases (PTPs), and its application in high-throughput determination of PTP substrate specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Biocatalysis*
Drug Design
Enzyme Inhibitors / pharmacology
Humans
Mutant Proteins / antagonists & inhibitors
Mutant Proteins / genetics*
Mutant Proteins / metabolism*
Mutation*
Phosphopeptides / chemistry
Phosphopeptides / metabolism*
Protein Array Analysis
Protein Tyrosine Phosphatases / antagonists & inhibitors
Protein Tyrosine Phosphatases / genetics*
Protein Tyrosine Phosphatases / metabolism*
Substrate Specificity

Substances

Enzyme Inhibitors
Mutant Proteins
Phosphopeptides
Protein Tyrosine Phosphatases