Biocompatibility evaluation of nano-rod hydroxyapatite/gelatin coated with nano-HAp as a novel scaffold using mesenchymal stem cells

J Biomed Mater Res A. 2010 Mar 15;92(4):1244-55. doi: 10.1002/jbm.a.32452.

Abstract

This study is devoted to fabricate a novel hydroxyapatite(HAp)/gelatin scaffold coated with nano-HAp in nano-rod configuration to evaluate its biocompatibility potential. The nano-HAp particles are needle and rod-like with widths ranging between 30 to 60 nm and lengths from 100 to 300 nm, respectively. Because of their higher surface area and higher reactivity, the nano-rod particles were distributed in gelatin much better than spherical and mixed shapes particles. The compressive modulus of the nano-HAp/gelatin scaffolds coated with nano-HAp was comparable with the compressive modulus of a human cancellous bone. The potential performance of the fabricated scaffolds as seeding media was assayed using mesenchymal stem cells (MSCs). MTT (3-(4,5-dimethylthiazol-2-yl)-1,5-diphenyl tetrazulium bromide) assays were performed on days 4 and 7 and the number of the cells per scaffold was determined. On the basis of this assay, all the studied scaffolds exhibited an appropriate environment in which the loaded cells appeared to be proliferated during the cultivation periods. In all fabricated composite scaffolds, marrow-derived MSCs appeared to occupy the scaffolds internal spaces and attach on their surfaces. According to the cell culture experiments, the incorporation of rod-like nano-HAp and coating of scaffolds with nano-HAp particles enabled the prepared scaffolds to possess desirable biocompatibility, high bioactivity, and sufficient mechanical strength in comparison with noncoated HAp samples. This research suggests that the newly developed scaffold has a potential as a suitable scaffold for bone tissue engineering.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Bone and Bones / anatomy & histology
  • Bone and Bones / physiology
  • Cell Culture Techniques
  • Cell Line
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / metabolism*
  • Durapatite / chemistry
  • Durapatite / metabolism*
  • Gelatin / chemistry
  • Gelatin / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Materials Testing
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology*
  • Mice
  • Nanostructures / chemistry*
  • Particle Size
  • Porosity
  • Rats
  • Rats, Wistar
  • Spectroscopy, Fourier Transform Infrared
  • Stress, Mechanical
  • Surface Properties
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry*

Substances

  • Coated Materials, Biocompatible
  • Gelatin
  • Durapatite