Abstract
This cross-sectional study investigated with two-dimensional gel electrophoresis coupled to MALDI-TOF and MRI the relationship between PBMCs protein expression profile and whole-brain atrophy in 16 unselected RR-MS IFN-treated patients compared with 6 RR IFN-untreated and 12 matched healthy control subjects. Grey/white matter fraction, T1/T2 lesion load and clinical variables were considered too. Twenty six proteins showed significant differential expression among RR IFN-treated patients and control samples. Four of these (IN35, GANAB, PP1B, SEPT2) resulted correlated with clinical and MRI findings in RR IFN-treated MS patients. Future clinical applications remain to be validated by other techniques and confirmed by a larger study.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Aged
-
Atrophy / pathology*
-
Atrophy / physiopathology
-
Brain / pathology*
-
Brain / physiopathology
-
Cross-Sectional Studies
-
Disease Progression
-
Electrophoresis, Gel, Two-Dimensional
-
Female
-
Humans
-
Leukocytes, Mononuclear / immunology
-
Leukocytes, Mononuclear / metabolism*
-
Magnetic Resonance Imaging
-
Male
-
Middle Aged
-
Multiple Sclerosis, Relapsing-Remitting / immunology
-
Multiple Sclerosis, Relapsing-Remitting / metabolism*
-
Multiple Sclerosis, Relapsing-Remitting / pathology*
-
Nerve Fibers, Myelinated / pathology
-
Phosphoric Monoester Hydrolases / analysis
-
Phosphoric Monoester Hydrolases / metabolism
-
Pilot Projects
-
Predictive Value of Tests
-
Proteomics / methods
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Young Adult
Substances
-
Phosphoric Monoester Hydrolases