High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis

J Biophotonics. 2008 Dec;1(6):514-21. doi: 10.1002/jbio.200810054.

Abstract

We report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM-FRET.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Line
  • Chlorocebus aethiops
  • Fluorescence Resonance Energy Transfer / instrumentation
  • Fluorescent Dyes
  • Humans
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Fluorescence / instrumentation*
  • Protein Interaction Mapping / instrumentation

Substances

  • Fluorescent Dyes