Abstract
Latex agglutination has been used to detect capsular polysaccharides from a variety of bacteria in body fluids. A latex agglutination assay was constructed for detection of the poly-gamma-D-glutamic acid (gammaDPGA) capsular polypeptide of Bacillus anthracis in serum from animal models of pulmonary anthrax. The assay was able to detect gammaDPGA in serum from infected animals at concentrations of 100 to 200 ng/mL.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Anthrax / diagnosis*
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Antigens, Bacterial / analysis*
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Antigens, Bacterial / immunology
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Bacillus anthracis / chemistry
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Bacillus anthracis / immunology
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Bacillus anthracis / isolation & purification*
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Bacterial Capsules / chemistry*
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Bacterial Capsules / immunology
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Latex Fixation Tests / methods
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Mice
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Mice, Inbred BALB C
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Polyglutamic Acid / analogs & derivatives*
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Polyglutamic Acid / analysis
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Polyglutamic Acid / immunology
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Serum / chemistry
Substances
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Antigens, Bacterial
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poly(gamma-glutamic acid)
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Polyglutamic Acid