Metabolite interference in pharmacokinetic studies

Drug Metab Lett. 2007 Dec;1(4):272-5. doi: 10.2174/187231207783221475.

Abstract

Our policy of conducting biotransformation studies with extended chromatography prior to pharmacokinetic bioanalyses allowed us to quickly detect an unusual, cis/trans metabolite in rat plasma that was inseparable using a short chromatographic method. We caution investigators that short methods invite unknown isobaric metabolites to cause inaccuracies in plasma concentration measurements.

MeSH terms

  • Animals
  • Biotransformation
  • Chromatography, Liquid / methods*
  • Cyclohexanols / metabolism*
  • Drug Discovery / methods
  • Female
  • Hepatocytes / metabolism
  • Male
  • Pharmaceutical Preparations / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Stereoisomerism

Substances

  • Cyclohexanols
  • Pharmaceutical Preparations