Parallel RNA-strand recognition by 2'-amino-beta-L-LNA

T Santhosh Kumar; Michael E Ostergaard; Pawan K Sharma; Poul Nielsen; Jesper Wengel; Patrick J Hrdlicka

doi:10.1016/j.bmcl.2009.03.079

Parallel RNA-strand recognition by 2'-amino-beta-L-LNA

Bioorg Med Chem Lett. 2009 May 1;19(9):2396-9. doi: 10.1016/j.bmcl.2009.03.079. Epub 2009 Mar 24.

Authors

T Santhosh Kumar¹, Michael E Ostergaard, Pawan K Sharma, Poul Nielsen, Jesper Wengel, Patrick J Hrdlicka

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark.

PMID: 19356930
DOI: 10.1016/j.bmcl.2009.03.079

Abstract

A short synthetic route to the first beta-l-ribo configured locked nucleic acid (LNA), that is, 2'-amino-beta-l-LNA thymine phosphoramidite 6, has been developed from bicyclic nucleoside 1. Incorporation of 2'-amino-beta-l-LNA thymine monomers into alpha-DNA strands results in probes forming stable duplexes with complementary RNA in parallel orientation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry
Hot Temperature
Models, Chemical
Molecular Structure
Nucleic Acid Conformation
Nucleic Acid Denaturation
Oligonucleotides / chemistry*
RNA / chemistry*
Temperature
Thymine / chemistry

Substances

Oligonucleotides
locked nucleic acid
RNA
DNA
Thymine