A comparative study of proliferation indices and ploidy in dysplastic naevi and malignant melanomas using flow cytometry

Histopathology. 1991 Oct;19(4):337-44. doi: 10.1111/j.1365-2559.1991.tb00048.x.

Abstract

Cell proliferation indices and DNA content have been determined in 18 intradermal naevi, 40 dysplastic naevi and 16 superficial malignant melanomas (less than 0.76 mm depth of invasion) using flow cytometry. In this study, proliferation indices of intradermal naevi and dysplastic naevi were not significantly different from each other. Abnormalities of DNA ploidy were not identified in the intradermal naevi or dysplastic naevi; whereas three of the malignant melanomas were aneuploid. In addition, cellular proliferation was increased within the group of malignant melanomas, in comparison with the naevi. This study has found no evidence to indicate that sporadic dysplastic naevi were more likely than intradermal naevi to transform to malignant melanoma, when objective criteria were employed. However, dysplastic naevi could be distinguished from some early malignant melanomas by absence of aneuploidy and by low cell proliferation indices.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Cell Division
  • Cell Transformation, Neoplastic
  • Child
  • DNA, Neoplasm / analysis*
  • Dysplastic Nevus Syndrome / genetics*
  • Dysplastic Nevus Syndrome / pathology*
  • Female
  • Flow Cytometry
  • Humans
  • Male
  • Melanoma / genetics*
  • Melanoma / pathology*
  • Middle Aged
  • Nevus, Pigmented / genetics
  • Nevus, Pigmented / pathology
  • Ploidies
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / pathology*

Substances

  • DNA, Neoplasm