Abstract
Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3'-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric oligonucleotide, but much higher resistance towards 3'-exonucleolytic degradation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA / chemistry*
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Exonucleases / metabolism
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Oligonucleotides / chemical synthesis
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Oligonucleotides / chemistry*
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Oligonucleotides / metabolism
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Oligonucleotides, Antisense / chemical synthesis
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Oligonucleotides, Antisense / chemistry*
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Oligonucleotides, Antisense / metabolism
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Organophosphates / chemistry*
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Phosphoric Diester Hydrolases / metabolism*
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RNA / chemistry
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Snakes
Substances
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Oligonucleotides
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Oligonucleotides, Antisense
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Organophosphates
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locked nucleic acid
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RNA
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methylphosphate
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DNA
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Exonucleases
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Phosphoric Diester Hydrolases