Abstract
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CHO Cells
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Cricetinae
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Cricetulus
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Drug Carriers / administration & dosage
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Drug Carriers / chemistry
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Fluorescent Dyes / administration & dosage
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Fluorescent Dyes / chemistry*
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Gene Products, tat / administration & dosage
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Gene Products, tat / chemistry*
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Lasers
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Photochemistry*
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RNA Interference*
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RNA, Small Interfering / administration & dosage*
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Recombinant Fusion Proteins / administration & dosage
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Recombinant Fusion Proteins / chemistry
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Ribonucleoprotein, U1 Small Nuclear / administration & dosage
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Ribonucleoprotein, U1 Small Nuclear / chemistry*
Substances
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Drug Carriers
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Fluorescent Dyes
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Gene Products, tat
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RNA, Small Interfering
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Recombinant Fusion Proteins
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Ribonucleoprotein, U1 Small Nuclear
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U1A protein