Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae

Alain R Bataille; François Robert

doi:10.1007/978-1-60327-015-1_18

Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae

Methods Mol Biol. 2009:543:267-79. doi: 10.1007/978-1-60327-015-1_18.

Authors

Alain R Bataille¹, François Robert

Affiliation

¹ Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, QC, Canada, H2W 1R7.

PMID: 19378172
DOI: 10.1007/978-1-60327-015-1_18

Abstract

Chromatin immunoprecipitation coupled to DNA microarray has become a widely used method to study transcription factors and chromatin structure. Here, we provide a detailed protocol for the localization of the variant histone Htz1 in the S. cerevisiae genome. This protocol can easily be adapted to fit other purposes such as profiling histone modifications.

MeSH terms

Chromatin / metabolism
Chromatin Immunoprecipitation / methods*
Cross-Linking Reagents / pharmacology
DNA, Fungal / isolation & purification
Genome, Fungal / genetics*
Histones / metabolism*
Mutation / genetics*
Oligonucleotide Array Sequence Analysis*
Polymerase Chain Reaction
Protein Processing, Post-Translational* / drug effects
Saccharomyces cerevisiae / drug effects
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Solubility / drug effects

Substances

Chromatin
Cross-Linking Reagents
DNA, Fungal
Histones