Abstract
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Buffers
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Chromatography, High Pressure Liquid
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DNA / isolation & purification
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DNA / metabolism*
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DNA-Directed RNA Polymerases / metabolism
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Electrophoresis, Polyacrylamide Gel
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Fluorescence Resonance Energy Transfer / methods*
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Fluorescent Dyes / metabolism
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Lasers
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Proteins / metabolism*
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Spectrophotometry, Ultraviolet
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Staining and Labeling
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Transcription, Genetic
Substances
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Buffers
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Fluorescent Dyes
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Proteins
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DNA
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DNA-Directed RNA Polymerases