Methicillin-resistant Staphylococcus aureus among a network of French private-sector community-based-medical laboratories

Med Mal Infect. 2009 May;39(5):311-8. doi: 10.1016/j.medmal.2009.03.007. Epub 2009 Apr 22.

Abstract

Objective: The aim of this study was to estimate the frequency of methicillin-resistant Staphylococcus aureus (MRSA) strains in the French community and the proportion of Panton-Valentine (PVL)-MRSA.

Design: A cross-sectional study was made during a 3-month period in 2003 through a network of private-sector, community-based medical laboratories selected throughout France: the Labville network. Each MRSA isolate was included and characterized by French National Reference Center for Staphylococci. The total number of S. aureus isolates was also collected.

Results: Among the 283 patients infected or colonized by MRSA, 166 (59%) were considered as healthcare-associated, 14 (5%) as nursing-associated and 39 (14%) as community-acquired. The proportion of methicillin resistance among S. aureus was 14%. Taking into account the sampling design, the incidence of MRSA cases in French outpatients was estimated to be 0.50 [CI95%: 0.41-0.60] per 10,000 inhabitants. The molecular analysis confirmed that 80.6% belong to the Lyon clone, the most prevalent hospital MRSA clone spreading in France and 10.6% to a closely related clone. An emerging MRSA clone containing the tst1 gene was detected in six patients and the PVL-positive ST80 clone only in one, 22-year-old, patient.

Conclusion: Most of MRSA cases diagnosed in the community in France, in 2003, were elderly with specific risk factors and harbored hospital strains. The prevalence of PVL-MRSA remained low.

MeSH terms

  • Blood / microbiology
  • Community-Acquired Infections / epidemiology*
  • Community-Acquired Infections / transmission
  • Feces / microbiology
  • Female
  • France / epidemiology
  • Humans
  • Incidence
  • Laboratories / standards*
  • Male
  • Methicillin-Resistant Staphylococcus aureus* / isolation & purification
  • Outpatients
  • Sex Ratio
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / transmission