Abstract
Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and preservation by freezing the cell suspensions in tryptic soy broth with 20% glycerol were found to be suitable preservation methods; all strains were alive for 3 years and had a minimum loss of LT production.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Toxins / biosynthesis*
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Bacterial Toxins / genetics
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Bacteriological Techniques
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Cryopreservation
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Culture Media
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Enterotoxins / biosynthesis*
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Enterotoxins / genetics
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli / metabolism*
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Escherichia coli Proteins*
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Evaluation Studies as Topic
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Humans
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Plasmids
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Preservation, Biological / methods*
Substances
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Bacterial Toxins
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Culture Media
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Enterotoxins
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Escherichia coli Proteins
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heat-labile enterotoxin, E coli