Altered expression of CRMPs in the brain of bovine spongiform encephalopathy-infected mice during disease progression

Brain Res. 2009 Mar 19:1261:1-6. doi: 10.1016/j.brainres.2009.01.006. Epub 2009 Jan 15.

Abstract

While recent studies suggest that synaptic alterations are first events in the mechanisms of prion-mediated neurodegeneration, little is known on the identity of the neuronal plasticity-related genes potentially concerned. Here the expression of 4 Collapsin Response Mediator Proteins (CRMPs), a family of signal transduction proteins involved in brain development and altered in Alzheimer's disease, was studied in the brain of C57Bl/6 mice infected with the BSE strain of prion agent, using RT-PCR and Western-blot methods. At the terminal stage of the disease, gene expression of each CRMP had decreased, while at the mid-stage of the disease only CRMP4 (mRNA and protein) expression had increased, concomitant to the start of PrP(Sc) accumulation in the brainstem. Altogether our findings picked out originally CRMPs, and especially CRMP4, as potential contributors to prion pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Brain Diseases / metabolism*
  • Brain Stem / metabolism*
  • Cattle
  • Disease Progression*
  • Encephalopathy, Bovine Spongiform / metabolism*
  • Female
  • Immunohistochemistry
  • Mice
  • Nerve Tissue Proteins / metabolism*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Staining and Labeling
  • Time Factors

Substances

  • Nerve Tissue Proteins
  • RNA, Messenger