Short-term administration of a combination of recombinant growth hormone and insulin-like growth factor-I induces anabolism in maintenance hemodialysis

J Clin Endocrinol Metab. 2009 Jul;94(7):2299-305. doi: 10.1210/jc.2008-2262. Epub 2009 Apr 28.

Abstract

Context: Resistance to GH and IGF-I is a significant complication of severe chronic kidney disease, which contributes to muscle wasting. Pharmacological doses of recombinant human (rh) GH or rhIGF-I have been proposed to treat this catabolic condition.

Objective: This study was undertaken to examine the potential additive anabolic effects of rhGH + rhIGF-I compared with rhIGF-I.

Design: We studied eight well-nourished hemodialysis patients in a random crossover design and compared the metabolic effects of a 3-d administration of moderate dose of rhIGF-I (40 microg/kg per 12h) with an association of rhIGF-I + rhGH (50 microg/kg/d). Leucine kinetics, plasma amino acids (AAs), serum insulin, and IGF binding proteins (IGFBP)-1 and -3 were measured.

Results: The net protein balance was not affected by rhIGF-I alone, whereas serum insulin and IGFBP-3 decreased (P < 0.05) and IGFBP-1 increased (P < 0.01). With the combination rhGH + rhIGF-I, an increase of IGFBP-3 (P < 0.01) and insulin (P < 0.01) as well as a decrease of IGFBP-1 (P < 0.01) occurred. Plasma essential AAs (P = 0.01) as well as the essential to nonessential AA ratio (P < 0.001) decreased. Whole-body protein net balance increased significantly (P < 0.05) with a 22% decrease in leucine oxidation and a 15% increase in nonoxidative leucine disposal.

Conclusions: In dialysis patients, rhIGF-I administration at a moderate dose has no protein metabolic effect, but the association with a moderate dose of rhGH is followed by a significant anabolic response.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blood Glucose / metabolism
  • Cross-Over Studies
  • Drug Administration Schedule
  • Drug Combinations
  • Female
  • Growth Hormone / administration & dosage*
  • Growth Hormone / blood
  • Humans
  • Insulin-Like Growth Factor Binding Proteins / blood
  • Insulin-Like Growth Factor I / administration & dosage*
  • Insulin-Like Growth Factor I / metabolism
  • Male
  • Metabolism / drug effects*
  • Middle Aged
  • Proteins / metabolism
  • Recombinant Proteins / administration & dosage
  • Renal Dialysis* / methods
  • Time Factors
  • Up-Regulation / drug effects

Substances

  • Blood Glucose
  • Drug Combinations
  • Insulin-Like Growth Factor Binding Proteins
  • Proteins
  • Recombinant Proteins
  • Insulin-Like Growth Factor I
  • Growth Hormone