Abstract
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Birds
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Influenza A virus / classification*
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Influenza A virus / genetics*
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Influenza A virus / isolation & purification
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Influenza in Birds / virology*
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Molecular Sequence Data
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Neuraminidase / genetics*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Viral Proteins / genetics*
Substances
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Viral Proteins
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NA protein, influenza A virus
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Neuraminidase
Associated data
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GENBANK/AB472013
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GENBANK/AB472014
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GENBANK/AB472015
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GENBANK/AB472016
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GENBANK/AB472017
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GENBANK/AB472018
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GENBANK/AB472019
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GENBANK/AB472020
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GENBANK/AB472021
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GENBANK/AB472022
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GENBANK/AB472023
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GENBANK/AB472024
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GENBANK/AB472025
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GENBANK/AB472026
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GENBANK/AB472027
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GENBANK/AB472028
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GENBANK/AB472029
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GENBANK/AB472030
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GENBANK/AB472031
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GENBANK/AB472032
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GENBANK/AB472033
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GENBANK/AB472034
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GENBANK/AB472035
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GENBANK/AB472036
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GENBANK/AB472037
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GENBANK/AB472038
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GENBANK/AB472039
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GENBANK/AB472040
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GENBANK/AB472051
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GENBANK/AB472052
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GENBANK/AB472053
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GENBANK/AB472054
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GENBANK/AB472055
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GENBANK/AB472056
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GENBANK/AB472057
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GENBANK/AB472058
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GENBANK/AB472059
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GENBANK/AB472060
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GENBANK/AB472061
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GENBANK/AB472062
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GENBANK/AB472063