Abstract
Classical conditioning of Hermissenda, involving paired light-rotation events, results in a 30-35% decrease in the levels of a 20-kDa G protein (cp20). To test whether a similar protein exists in vertebrates, rabbits were trained to associate a tone with periorbital electrical stimulation and G proteins were analyzed by photoaffinity labeling with [alpha-32P]GTP-azidoanilide. A 20-kDa G protein similar to cp20 decreased by 36% in the hippocampus of rabbits subjected to paired tone and electrical stimulation, but not in unpaired controls. Learning-specific decreases were also found in the amount of ras protein.
MeSH terms
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Acoustic Stimulation
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Affinity Labels
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Animals
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Azides / metabolism
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Conditioning, Classical / physiology*
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Electric Stimulation
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Electrophoresis, Gel, Two-Dimensional
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Electrophoresis, Polyacrylamide Gel
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GTP-Binding Proteins / chemistry
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GTP-Binding Proteins / metabolism*
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GTPase-Activating Proteins
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Guanosine Triphosphate / analogs & derivatives
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Guanosine Triphosphate / metabolism
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Hippocampus / metabolism*
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Male
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Molecular Weight
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Osmolar Concentration
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Proteins / metabolism
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Rabbits
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ras GTPase-Activating Proteins
Substances
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Affinity Labels
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Azides
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GTPase-Activating Proteins
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Proteins
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ras GTPase-Activating Proteins
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GTP gamma-4-azidoanilide
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Guanosine Triphosphate
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GTP-Binding Proteins