Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

Ya-Yun Wang; Yan-Yan Wei; Jing Huang; Wei Wang; Nobuaiki Tamamaki; Yun-Qing Li; Sheng-Xi Wu

doi:10.1016/j.jchemneu.2009.04.003

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

J Chem Neuroanat. 2009 Sep;38(1):75-81. doi: 10.1016/j.jchemneu.2009.04.003. Epub 2009 May 3.

Authors

Ya-Yun Wang¹, Yan-Yan Wei, Jing Huang, Wei Wang, Nobuaiki Tamamaki, Yun-Qing Li, Sheng-Xi Wu

Affiliation

¹ Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.

PMID: 19409978
DOI: 10.1016/j.jchemneu.2009.04.003

Abstract

5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD(67)-GFP knock-in mice. The present result showed that 5-HT(1A) receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT(1A) receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT(2A) receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT(2A) receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Fluorescent Antibody Technique
Gene Expression Regulation / physiology
Gene Knock-In Techniques
Glutamate Decarboxylase / genetics*
Green Fluorescent Proteins / genetics
Interneurons / cytology
Interneurons / metabolism*
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neural Inhibition / genetics
Nociceptors / cytology
Nociceptors / metabolism
Pain / genetics
Pain / metabolism
Pain / physiopathology
Posterior Horn Cells / cytology
Posterior Horn Cells / metabolism*
Receptor, Serotonin, 5-HT1A / genetics
Receptor, Serotonin, 5-HT1A / metabolism*
Receptor, Serotonin, 5-HT2A / genetics
Receptor, Serotonin, 5-HT2A / metabolism
Recombinant Fusion Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction
Serotonin / metabolism
Synaptic Transmission / genetics
gamma-Aminobutyric Acid / metabolism*

Substances

Receptor, Serotonin, 5-HT2A
Recombinant Fusion Proteins
Receptor, Serotonin, 5-HT1A
Green Fluorescent Proteins
Serotonin
gamma-Aminobutyric Acid
Glutamate Decarboxylase
glutamate decarboxylase 1