Dietary omega-3 fatty acids attenuate cellular damage after a hippocampal ischemic insult in adult rats

Júlia D Moreira; Luisa Knorr; Ana Paula Thomazi; Fabrício Simão; Cíntia Battú; Jean Pierre Oses; Carmem Gottfried; Susana Wofchuk; Christianne Salbego; Diogo O Souza; Marcos L S Perry; Lúcia Vinadé

doi:10.1016/j.jnutbio.2009.01.013

Dietary omega-3 fatty acids attenuate cellular damage after a hippocampal ischemic insult in adult rats

J Nutr Biochem. 2010 Apr;21(4):351-6. doi: 10.1016/j.jnutbio.2009.01.013. Epub 2009 May 1.

Authors

Júlia D Moreira¹, Luisa Knorr, Ana Paula Thomazi, Fabrício Simão, Cíntia Battú, Jean Pierre Oses, Carmem Gottfried, Susana Wofchuk, Christianne Salbego, Diogo O Souza, Marcos L S Perry, Lúcia Vinadé

Affiliation

¹ Biochemistry Department, Biochemistry Postgraduate Program, ICBS, Federal University of Rio Grande do Sul, 90035-003 Porto Alegre, RS, Brazil. [email protected]

PMID: 19410444
DOI: 10.1016/j.jnutbio.2009.01.013

Abstract

The role of omega-3 polyunsaturated fatty acids (3PUFAs) on brain function is increasingly demonstrated. Here, the effect of dietary deprivation of essential 3PUFAs on some parameters related to neuroprotection was investigated. Rats were fed with two different diets: omega-3 diet and omega-3-deprived diet. To assess the influence of 3PUFAs on brain responses to ischemic insult, hippocampal slices were subjected to an oxygen and glucose deprivation (OGD) model of in vitro ischemia. The omega-3-deprived group showed higher cell damage and stronger decrease in the [(3)H]glutamate uptake after OGD. Moreover, omega-3 deprivation influenced antiapoptotic cell response after OGD, affecting GSK-3beta and ERK1/2, but not Akt, phosphorylation. Taken together, these results suggest that 3PUFAs are important for cell protection after ischemia and also seem to play an important role in the activation of antiapoptotic signaling pathways.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Death*
Cell Hypoxia
Dietary Fats, Unsaturated / administration & dosage*
Extracellular Signal-Regulated MAP Kinases / metabolism
Fatty Acids, Essential / deficiency
Fatty Acids, Omega-3 / administration & dosage*
Fatty Acids, Omega-3 / physiology
Female
Glycogen Synthase Kinase 3 / metabolism
Glycogen Synthase Kinase 3 beta
Hippocampus / metabolism
Hippocampus / physiopathology*
Hypoxia-Ischemia, Brain / metabolism
Hypoxia-Ischemia, Brain / physiopathology*
In Vitro Techniques
Male
Neuroprotective Agents / administration & dosage*
Phosphorylation
Protein Processing, Post-Translational
Rats
Rats, Wistar
Reperfusion Injury / metabolism
Reperfusion Injury / prevention & control*
Signal Transduction

Substances

Dietary Fats, Unsaturated
Fatty Acids, Essential
Fatty Acids, Omega-3
Neuroprotective Agents
Glycogen Synthase Kinase 3 beta
Gsk3b protein, rat
Extracellular Signal-Regulated MAP Kinases
Glycogen Synthase Kinase 3