Isolation, culture and characterization of adult carotid body-derived cells

Respir Physiol Neurobiol. 2009 Jun 30;167(2):201-7. doi: 10.1016/j.resp.2009.04.021. Epub 2009 May 3.

Abstract

Recent studies indicate that carotid body (CB) could be a suitable cell source for cell therapy in Parkinson's disease. We have isolated and successfully expanded in culture as monolayer adult CB-derived cells using a modification of the culture medium employed for bone marrow multipotent adult progenitor cells (MAPCs). These cells express variable amounts of tyrosine hydroxylase (TH), beta-III tubulin and Sox2. In addition, CB-derived cells showed high expression of Sox2 related to a high rate of proliferation and consistent with an undifferentiated state. Under culture conditions that reduced cell proliferation, Sox2 expression decreased while TH and beta-III tubulin expression was increased. This could indicate that the differentiation of some cells occurs in the culture, thus accounting for a certain neural differentiation potential of CB-derived cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Carotid Body / cytology*
  • Carotid Body / metabolism
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Proliferation
  • Male
  • Neurons / cytology*
  • Neurons / metabolism
  • Rats
  • Rats, Wistar
  • Stem Cells / cytology*
  • Stem Cells / metabolism