Abstract
HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (K(d)) of 10 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / biosynthesis*
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Carrier Proteins / isolation & purification*
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Carrier Proteins / metabolism
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Gene Expression
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Heme-Binding Proteins
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Hemeproteins / biosynthesis*
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Hemeproteins / isolation & purification*
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Hemeproteins / metabolism
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Periplasm*
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Photobacterium / cytology*
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / isolation & purification*
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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Heme-Binding Proteins
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Hemeproteins
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Recombinant Proteins