Abstract
Five new 7-O-methylkaempferol and -quercetin glycosides, namely, nervilifordins A-E (1-5), were isolated from the whole plant of Nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). Their structures were elucidated on the basis of extensive spectroscopic analyses, including HSQC, HMBC, ROESY, and chemical methods. Compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (HSV-1) activity and cytotoxicity on African green monkey kidney cells (Vero cells) in vitro. Of the tested compounds, only esculetin (8) exhibited antiviral activity against HSV-1, while the aglycones (11-13) showed stronger cytotoxicity on Vero cells than their glycosides (1-3, 6, and 7).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / chemistry
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Antiviral Agents / isolation & purification*
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Antiviral Agents / pharmacology
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Chlorocebus aethiops
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology
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Herpesvirus 1, Human / drug effects*
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Orchidaceae / chemistry*
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Plants, Medicinal / chemistry*
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Quercetin / chemistry
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Quercetin / isolation & purification*
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Quercetin / pharmacology
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Umbelliferones / chemistry
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Umbelliferones / isolation & purification*
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Umbelliferones / pharmacology
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Vero Cells
Substances
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Antiviral Agents
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Drugs, Chinese Herbal
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Glycosides
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Umbelliferones
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Quercetin
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esculetin