Abstract
We have investigated the effect of adiponectin (APN) peptide II on new vessel growth in mouse model of choroidal neovascularization (CNV) or wet type age-related macular degeneration (AMD). Mice were injected intraperitoneally with APN peptide II, control peptide, or PBS on day 1-7 or day 5-14. APN, AdipoR1, PCNA, and VEGF localization was investigated using confocal microscopy, immunohistochemistry, and RT-PCR. APN peptide II decreased the relative area of FITC-dextran perfused vessels by 4-fold, PCNA expression by 3-fold, and the number of PCNA stained HUVEC and MAVEC cells by 38 and 46%, respectively. We concluded that APN peptide II inhibits CNV size on days 7 and 14 by inhibiting the proliferation of endothelial cells in vivo and in vitro. APN peptide II may have therapeutic potential to inhibit CNV or wet AMD.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adiponectin / chemistry
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Adiponectin / therapeutic use*
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Amino Acid Sequence
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Animals
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Choroidal Neovascularization / drug therapy*
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Choroidal Neovascularization / etiology
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Choroidal Neovascularization / metabolism
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Endothelial Cells / metabolism
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Endothelial Cells / radiation effects
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Lasers
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Male
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Mice
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Mice, Inbred C57BL
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Models, Animal
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Peptide Fragments / chemistry
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Peptide Fragments / therapeutic use*
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Proliferating Cell Nuclear Antigen / metabolism
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Proliferating Cell Nuclear Antigen / physiology
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Protein Structure, Tertiary
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Receptors, Adiponectin / metabolism
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Receptors, Adiponectin / physiology
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Vascular Endothelial Growth Factor A / metabolism
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Vascular Endothelial Growth Factor A / physiology
Substances
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Adiponectin
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Peptide Fragments
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Proliferating Cell Nuclear Antigen
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Receptors, Adiponectin
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Vascular Endothelial Growth Factor A