Abstract
Blockage of Ca2+ uptake with thapsigargin, a specific antagonist of sarco/endoplasmic reticulum Ca2+-ATPase pumps, causes an increase of somatic Ca2+, with negligible changes of Ca2+ levels in dendrites. Treatment with thapsigargin in the presence of blockers of NMDA (N-methyl-D-aspartic acid) receptors upregulates some activity-dependent genes (Egr2 and Nr4a1), leaving unaltered the expression level of other activity-dependent genes (Bdnf and Arc). These results show that the elevation of somatic Ca2+ can initiate transcription of specific genes, independently of activation of NMDA receptors, but that transcription of other genes is not initiated by a simple elevation of intracellular Ca2+.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Brain-Derived Neurotrophic Factor / drug effects
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Brain-Derived Neurotrophic Factor / metabolism
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Calcium / metabolism*
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Calcium Signaling / drug effects
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Calcium Signaling / physiology*
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Calcium-Transporting ATPases / antagonists & inhibitors
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Calcium-Transporting ATPases / metabolism
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Cytoskeletal Proteins / drug effects
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Cytoskeletal Proteins / metabolism
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / metabolism*
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Early Growth Response Protein 2 / drug effects
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Early Growth Response Protein 2 / metabolism*
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Endoplasmic Reticulum / drug effects
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Endoplasmic Reticulum / metabolism
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Enzyme Inhibitors / pharmacology
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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Hippocampus / ultrastructure
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Nerve Tissue Proteins / drug effects
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Nerve Tissue Proteins / metabolism
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Organ Culture Techniques
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Patch-Clamp Techniques
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Pyramidal Cells / drug effects
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Pyramidal Cells / metabolism*
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Pyramidal Cells / ultrastructure
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Rats
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Rats, Wistar
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Receptors, Steroid / drug effects
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Receptors, Steroid / metabolism*
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Thapsigargin / pharmacology
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Transcriptional Activation / drug effects
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Transcriptional Activation / physiology
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Up-Regulation / drug effects
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Up-Regulation / physiology
Substances
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Brain-Derived Neurotrophic Factor
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Cytoskeletal Proteins
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DNA-Binding Proteins
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Early Growth Response Protein 2
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Egr2 protein, rat
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Enzyme Inhibitors
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Nerve Tissue Proteins
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Nr4a1 protein, rat
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Nuclear Receptor Subfamily 4, Group A, Member 1
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Receptors, Steroid
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activity regulated cytoskeletal-associated protein
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Thapsigargin
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Calcium-Transporting ATPases
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Calcium