A chemoenzymatic route to N-acetylglucosamine-1-phosphate analogues: substrate specificity investigations of N-acetylhexosamine 1-kinase

Chem Commun (Camb). 2009 May 28:(20):2944-6. doi: 10.1039/b904853g. Epub 2009 Apr 24.

Abstract

Reports an efficient chemoenzymatic production of an N-acetylhexosamine 1-phophate analogues library by N-acetylhexosamine 1-kinase (NahK) and describes the respective substrate specificity on this enzyme.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylglucosamine / analogs & derivatives*
  • Acetylglucosamine / chemical synthesis
  • Acetylglucosamine / chemistry
  • Acetylglucosamine / metabolism
  • Adenosine Triphosphate / chemical synthesis
  • Adenosine Triphosphate / metabolism
  • Bifidobacterium / enzymology*
  • Isomerism
  • Phosphotransferases / chemical synthesis
  • Phosphotransferases / metabolism*
  • Small Molecule Libraries / chemical synthesis*
  • Small Molecule Libraries / chemistry
  • Small Molecule Libraries / metabolism*
  • Substrate Specificity

Substances

  • Small Molecule Libraries
  • N-acetylglucosamine-1-phosphate
  • Adenosine Triphosphate
  • Phosphotransferases
  • Acetylglucosamine