Electrostatically mediated liposome fusion and lipid exchange with a nanoparticle-supported bilayer for control of surface charge, drug containment, and delivery

J Am Chem Soc. 2009 Jun 10;131(22):7567-9. doi: 10.1021/ja902039y.

Abstract

The loading and containment of cargo within nanoparticles and their efficient delivery to cells represent a primary challenge in nanomedicine. We report lipid exchange between free and mesoporous silica nanoparticle-supported lipid bilayers as an effective means of containing cargo, controlling charge, and directing delivery to mammalian cells. The delivery of a membrane-impermeable dye (calcein) and a chemotherapeutic drug (doxorubicin) are demonstrated. Exchanged lipid bilayers minimized premature drug release, and an overall positive charge on the supported lipid bilayer effected enhanced delivery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Doxorubicin / administration & dosage
  • Doxorubicin / chemistry
  • Drug Delivery Systems / methods*
  • Fatty Acids, Monounsaturated / chemistry
  • Fluoresceins / administration & dosage
  • Fluoresceins / chemistry
  • Fluoresceins / pharmacokinetics
  • Lipid Bilayers / chemistry*
  • Liposomes / administration & dosage
  • Liposomes / chemistry*
  • Nanoparticles / administration & dosage
  • Nanoparticles / chemistry*
  • Quaternary Ammonium Compounds / chemistry
  • Silicon Dioxide / chemistry
  • Spectrometry, Fluorescence
  • Static Electricity
  • Surface Properties

Substances

  • Fatty Acids, Monounsaturated
  • Fluoresceins
  • Lipid Bilayers
  • Liposomes
  • Quaternary Ammonium Compounds
  • Silicon Dioxide
  • Doxorubicin
  • 1,2-dioleoyloxy-3-(trimethylammonium)propane
  • fluorexon