Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells

J Am Chem Soc. 2009 Jun 17;131(23):8102-7. doi: 10.1021/ja900225w.

Abstract

Quantum dots multifunctionalized with the amyloid protein alpha-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only approximately 100 nanoparticles. The combination with a tetracysteine-tagged form of alpha-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / chemistry*
  • Amyloid / metabolism*
  • Biosensing Techniques*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Microscopy, Atomic Force
  • Microscopy, Fluorescence
  • Quantum Dots*
  • alpha-Synuclein / chemistry

Substances

  • Amyloid
  • Fluorescent Dyes
  • alpha-Synuclein