Abstract
The two forms of RNA polymerase II that exist in vivo, phosphorylated (IIO) and nonphosphorylated (IIA), were purified to apparent homogeneity from HeLa cells. The nonphosphorylated form preferentially binds to the preinitiation complex. RNA polymerase II in the complex was converted by a cellular protein kinase to the phosphorylated form.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Chromatography, DEAE-Cellulose
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Chromatography, High Pressure Liquid
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DNA, Viral / metabolism*
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HeLa Cells
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Humans
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Isoenzymes / isolation & purification
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Isoenzymes / metabolism*
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Kinetics
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Macromolecular Substances
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Molecular Weight
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Phosphorylation
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Promoter Regions, Genetic
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Protein Binding
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Protein Kinases / metabolism*
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RNA Polymerase II / isolation & purification
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RNA Polymerase II / metabolism*
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
Substances
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DNA, Viral
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Isoenzymes
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Macromolecular Substances
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Recombinant Proteins
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Transcription Factors
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Protein Kinases
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RNA Polymerase II