Abstract
The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aquaporin 4 / genetics
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Aquaporin 4 / metabolism
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Aquaporins / genetics
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Aquaporins / physiology*
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Biotinylation
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COS Cells
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Cell Adhesion / physiology*
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Cell Membrane / metabolism
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Chlorocebus aethiops
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Drosophila Proteins / genetics
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Drosophila Proteins / physiology*
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Gene Expression
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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L Cells
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Mice
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Patch-Clamp Techniques
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
Substances
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AQP4 protein, human
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Aquaporin 4
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Aquaporins
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Drosophila Proteins
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Membrane Proteins
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Recombinant Fusion Proteins
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bib protein, Drosophila
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enhanced green fluorescent protein
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Green Fluorescent Proteins