No detection of human bocavirus in amniotic fluid samples from fetuses with hydrops or isolated effusions

J Clin Virol. 2009 Aug;45(4):300-3. doi: 10.1016/j.jcv.2009.04.005. Epub 2009 May 26.

Abstract

Background: Human bocavirus (HBoV) is a recently identified parvovirus associated with respiratory disease in infants. Animal bocaviruses have been shown to cause intrauterine infection, fetal anasarca and abortion in late gestation.

Objectives: To investigate whether HBoV infection is associated with fetal hydrops, fetal anemia or isolated fetal effusions.

Study design: We determined the prevalence of HBoV and parvovirus B19 (B19) DNA in amniotic fluid samples from fetuses with hydrops, anemia or isolated effusions using different real-time PCR protocols, and the HBoV IgG and IgM positivity rate in pregnant women with fetal hydrops or normal ultrasound findings by a non-commercial virus-like particle-based enzyme immunoassay.

Results: None of 87 amniotic fluid samples tested was HBoV DNA positive. Twelve of 60 fetuses with hydrops or anemia were found B19 DNA positive. Anti-HBoV IgG antibodies were detected in 100% (19/19) and 94% (47/50) of serum samples from pregnant women with fetal hydrops and normal ultrasound findings, respectively. All serum samples were found negative for anti-HBoV IgM.

Conclusion: We suggest that HBoV is not a common cause of fetal hydrops, anemia or isolated effusions. This has to be confirmed by further studies of proven gestational HBoV infection.

MeSH terms

  • Adult
  • Amniotic Fluid / virology*
  • Anemia / virology*
  • Animals
  • Antibodies, Viral / blood
  • Bocavirus / isolation & purification*
  • Edema / virology*
  • Female
  • Fetal Diseases / virology*
  • Fetus / virology*
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Parvoviridae Infections / virology*
  • Parvovirus B19, Human / isolation & purification
  • Polymerase Chain Reaction / methods
  • Pregnancy
  • Pregnancy Complications, Infectious / virology
  • Prevalence

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M