Grafting of silica with sulfobetaine polymers via aqueous reversible addition fragmentation chain transfer polymerization and its use as a stationary phase in HILIC

J Sep Sci. 2009 Jun;32(12):2008-16. doi: 10.1002/jssc.200800554.

Abstract

Porous silica particles of 3 microm diameter and 100 A nominal pore size were first activated for vinylic polymerization by functionalization with 3-methacryloyloxypropyl trimethoxysilane (MAPTMS) and thereafter dressed with zwitterionic grafts of the sulfoalkylbetaine type in the "grafting through" fashion by polymerizing 3-(2-(N-methacryloyloxyethyl)-N,N-dimethylammonio)propane sulfonate (SPE), using either free radical polymerization or controlled reversible addition fragmentation chain transfer polymerization (RAFT). Particles polymerized using RAFT had a lower overall coating which seemed to be more evenly distributed in the pore volume. Both approaches resulted in columns with similar separation properties in HILIC mode.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid / instrumentation*
  • Chromatography, High Pressure Liquid / methods
  • Materials Testing
  • Methacrylates / chemistry
  • Molecular Sequence Data
  • Molecular Structure
  • Nitrogen / chemistry
  • Particle Size
  • Peptides / analysis
  • Peptides / genetics
  • Polymers / chemical synthesis
  • Polymers / chemistry*
  • Porosity
  • Silanes / chemistry
  • Silicon Dioxide / chemistry*
  • Surface Properties

Substances

  • 3-methacryloxypropyltrimethoxysilane
  • Methacrylates
  • Peptides
  • Polymers
  • Silanes
  • Silicon Dioxide
  • Nitrogen